The Lipid and Lipid Metabolite Analysis Core Facility is a joint initiative of the Group on the Molecular and Cell Biology of Lipids (MCBL) and the Cardiovascular Research Group (CVRG) with support from the Mazankowski Alberta Heart Institute (MAHI) and the Women and Children's Health Research Institute (WCHRI).

Services currently available:

1. FPLC lipoprotein profiling from various sources including plasma, cell culture medium, and isolated intracellular compartments (cholesterol/triglyceride/phospholipid profiles)
2. FPLC separation and purification of lipoprotein classes and subclasses including VLDL, LDL, and HDL
3. HPLC analysis and quantitation of phospholipid classes from various sources including tissue, plasma, and cell extracts
4. HPLC analysis and quantitation of bile acids from various sources including isolated bile
5. HPLC analysis of nucleotides including xanthine, hypoxanthine, inosine, IMP, NAD, adenosine, AMP, ADP, ATP, and GTP
6. HPLC analysis of short/long Chain CoA, Free CoA, Acetyl CoA, Malonyl CoA and Succinyl CoA, Palmitoyl CoA, Stearoyl CoA and Oleoyl CoA
7. HPLC analysis of ceramides
8. GC analysis of total lipids
9. GC analysis of fatty acid chain length and saturation
10. customized HPLC or FPLC protein purification*
11. customized HPLC or FPLC lipid analysis*
12. customized analysis of other molecules and metabolites*

Services to be available in the near future:

1. HPLC analysis of total lipid classes from various sources
2. customized metabolite analysis using the Luminex system
3. mass spectrometry (alone or coupled to HPLC separation)

*customized services will involve discussion with clients regarding materials and methods required for analysis